Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

CircZNF652 accelerates the proliferation and migration of primary lung carcinoma cells by downregulating miR-766

Xiaobing Hong , Yongqing Chen, Danzhen Zhang

Department of Respiratory and Critical Care Medicine, Taizhou Hospital of Traditional Chinese Medicine, Taizhou, China;

For correspondence:- Xiaobing Hong Email: 2329418023@qq.com Tel:+86057688825615

Accepted: 2 October 2021 Published: 30 November 2021

Citation: Hong X, Chen Y, Zhang D. CircZNF652 accelerates the proliferation and migration of primary lung carcinoma cells by downregulating miR-766. Trop J Pharm Res 2021; 20(11):2255-2260 doi: 10.4314/tjpr.v20i11.3

© 2021 The authors.
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Abstract

Purpose: To explore the biological functions and molecular mechanism of circZNF652 involvement in primary lung carcinoma.

Methods: CircZNF652 levels in primary lung carcinoma cases and controls were determined using quantitative real-time polymerase chain reaction (qRT-PCR). Its prognostic value in primary lung carcinoma was examined by depicting it with Kaplan-Meier curves. The biological functions of circZNF652 in regulating proliferative and migratory capacities in A549 and SPC-A-1 cells were analyzed from the curves. Interaction between circZNF652 and its downstream gene, miR-766, was assessed, and their co-regulation on primary lung carcinoma was determined by rescue experiments.

Results: CircZNF652 was abnormally and significantly upregulated in primary lung carcinoma cases (p < 0.05), resulting in a poor prognosis. The knockdown effect of circZNF652 attenuated the proliferative and migratory capacities of A549 and SPC-A-1 cells, and downregulated epithelial-mesenchymal transition (EMT)-associated genes. CircZNF652 bound and negatively regulated miR-766, a key downstream gene involved in circZNF652-induced aggravation of primary lung carcinoma.

Conclusion: CircZNF652 serves as an oncogene, triggering the aggravation of primary lung carcinoma by negatively regulating miR-766. The results of this study may provide new insights into the treatment of lung carcinoma.

Keywords: CircZNF652, Primary lung carcinoma, MiR-766, Epithelial-mesenchymal transition